“Bioline,siRNA转染试剂,假病毒包装,荧光定量PCR试剂,唾液DNA提取试剂”/

“Bioline,siRNA转染试剂,假病毒包装,荧光定量PCR试剂,唾液DNA提取试剂”/
核酸转染
当前位置:首页 > 科研试剂 > 核酸转染 > 正文

RFect siRNA/miRNA Transfection Reagent

RFect小核酸转染试剂

货号规格(ml)目录价(元)

 网购价(元)

(电议)

110110.51,080电议
1101211,830电议
110131.52,590电议

产品简介

RFect siRNA转染试剂是一种采用新型的动物源性的纳米材料,毒性很低并且拥有非常好的转染性能的一种小核酸转染试剂。RFect可用来转染siRNA、antisense RNA、microRNA等200bp以内的小分子RNA和DNA。RFect使用极其简便,先将siRNA与RFect室温混合,再将siRNA-RFect 混合物直接加入含培养基的细胞,血清对转染效果没有影响,不必刻意添加或更换培养液。有关RFect siRNA转染试剂的材料合成和试剂配制我们已申请了国际专利,并通过PCT覆盖多个国家和地区。

产品特点

· 很好的细胞转染性能:细胞转染阳性率高达90%以上,基因敲除效果明显,A549细胞LaminA/C基因敲除效率在95%以上;

· 极低的细胞毒性使实验结果更为客观:转染细胞死亡率不到10%;

· 低浓度siRNA转染获得高基因抑制水平;

· 转染细胞范围广,绝大多数贴壁细胞株都能获得比较理想的转染结果(如一般细胞株、肿瘤细胞株等)。

 

 

 

 

 

 

 

 

Fig. 1. RFect sRNA transfection reagent offers the highest transfection efficiency.  HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using RFect sRNA transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

 

 

 

 

 

 

 

 

Fig..2. RFect siRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFect  In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

 

 Fig..3.RFect siRNA transfection reagent offers high level of gene knockdown with low toxicity. A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using RFect siRNA transfection reagent. RFect concentrations added to the cells  exceeding transfection concentration of 20 times, the cells still do not produce significant toxicity, which makes these product suitable to optimization. Lamin A/C knockdown was measured by qRT-PCR.

 

Fig.4.RFect siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using RFect.


适用细胞

293T(人肾上皮细胞),A549(人肺癌细胞),HEK 293(人胚肾细胞),HeLa(人宫颈癌细胞),MCF-7(人乳腺癌细胞),HepG2(人肝肿瘤细胞),HT-1080(人纤维肉瘤),HLF-a(人肺细胞),Huh-7(人肝癌细胞),KB(人口腔表皮样癌细胞),PC3(人前列腺癌细胞),BRL-3A(大鼠正常肝细胞),DU 145(人前列腺癌细胞),Hepa 1-6(小鼠肝癌细胞),OVCAR3(人卵巢癌细胞),NCaP-FGC(人前列腺癌细胞),C2C12(成肌细胞),MDCK(犬肾细胞),CHO-K1,islk.219细胞(人肺癌细胞),HT-29(人结肠癌细胞),NCI-H1688(人典型小细胞肺肿瘤/癌细胞),RKO结肠癌细胞,Vero(非洲绿猴肾细胞),COS-1(非洲绿猴肾成纤维细胞),COS7-L(非洲绿猴肾成纤维细胞),COS-7(非洲绿猴肾成纤维细胞),SK-BR3(人乳腺癌细胞),Hepa 1cLc7(鼠肝癌细胞),Daoy(人髓母细胞瘤细胞),DBTRG-05MG(人脑胶质母细胞瘤细胞),KLN 205(鼠肺磷癌细胞),Neuro-2a(小鼠神经瘤母细胞),DI-TNC1(大鼠脑间质细胞)等。


相关文献

1.   Basic Research in Cardiology(2021). HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype. (IF:11.981) DOI: 10.1007/s00395-021-00867-7

2.   NUCLEIC ACIDS RESEARCH(2021). XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage. (IF:11.24 ) DOI: 10.1093/nar/gkv472

3.   AGING-US(2021). Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury. (IF:4.909 ) DOI: 10.18632/aging.202611

4.   International Journal of Biological Sciences(2021). DGCR5 Promotes Gallbladder Cancer by Sponging MiR-3619-5p via MEK/ERK1/2 and JNK/p38 MAPK Pathways. (IF:4.773) DOI: 10.7150/jca.46351

5.   International Journal of Biological Sciences(2021). Rac GTPase activating protein 1 promotes gallbladder cancer via binding DNA ligase 3 to reduce apoptosis. (IF:4.773) DOI: 10.7150/ijbs.58857

6.   International Journal of Biological Sciences(2021). ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. (IF:4.773)  DOI:10.7150/ijbs.57623

7.   International Journal of Biological Sciences(2021). KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. (IF:4.773 ) DOI: 10.7150/ijbs.54074

8.   scientific reports(2021). Clinical significance of Annexin A2 e­xpression in oral squamous cell carcinoma and its influence on cell proliferation, migration and invasion. (IF:4.149) DOI: 10.1038/s41598-021-84675-y

9.   Journal of Cancer(2021). LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1. (IF:3.565) DOI: 10.7150/jca.51136

10.   Environmental Geochemistry and Health(2021). Inorganic arsenic influences cell apoptosis by regulating the e­xpression of MEG3 gene. (IF:3.472 ) DOI: 10.1007/s10653-020-00740-x

11.   Molecular Human Reproduction(2021). Excess fibronectin 1 participates in pathogenesis of pre-eclampsia by promoting apoptosis and autophagy in vascular endothelial cells. (IF:3.469) DOI: 10.1093/molehr/gaab030

12.   American Journal of Translational Research(2021). Irg1-itaconate axis protects against acute kidney injury via activation of Nrf2. (IF:3.375 )

13.   ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2021). Arsenic exposure increased e­xpression of HOTAIR and LincRNA-p21 in vivo and vitro. (IF:3.33) DOI: 10.1007/s11356-020-10487-8

14.   Environmental Toxicology and Pharmacology(2021). Long non-coding RNA DICER1-AS1-low e­xpression in arsenic-treated A549 cells inhibits cell proliferation by regulating the cell cycle pathway. (IF:3.292 ) DOI: 10.1016/j.etap.2021.103617

15.   ENVIRONMENTAL TOXICOLOGY(2021). Inorganic arsenic‐mediated upregulation of AS3MT promotes proliferation of nonsmall cell lung cancer cells by regulating cell cycle genes. (IF:3.118 ) DOI: 10.1002/tox.23026

16.   FEBS Open Bio(2021). SREBP1/FASN/cholesterol axis facilitates radioresistance in colorectal cancer.  (IF:2.231 ) DOI: 10.1002/2211-5463.13137

17.   Clinical And Translational Medicine(2020). Long noncoding RNA lncGALM increases risk of liver metastasis in gallbladder cancer through facilitating N‐cadherin and IL‐1β‐dependent liver arrest and tumor extravasation. (IF:7.919 ) DOI: 10.1002/ctm2.201

18.   Clinical And Translational Medicine(2020). Mir‐27a‐3p attenuates bronchiolitis obliterans in vivo via the regulation of dendritic cells' maturation and the suppression of myofibroblasts' differentiation. (IF:7.919 ) DOI: 10.1002/ctm2.140

19.   Journal of Experimental & Clinical Cancer Research(2020). Loss of miR-192-5p initiates a hyperglycolysis and stemness positive feedback in hepatocellular carcinoma. (IF:7.068) DOI: 10.1186/s13046-020-01785-7

20.   American Journal of Cancer Research(2020). ERRα promotes pancreatic cancer progression by enhancing the transcription of PAI1 and activating the MEK/ERK pathway.  (IF:5.884 )

21.   Frontiers in Cell and Developmental Biology(2020). CRLF1 is a key regulator in the ligamentum flavum hypertrophy. (IF:5.8 ) DOI: 10.3389/fcell.2020.00858

22.   Cancer Science(2020). Anthrax toxin receptor 1/tumor endothelial marker 8 promotes gastric cancer progression through activation of the PI3K/AKT/mTOR signaling pathway. (IF:4.985) DOI: 10.1111/cas.14326

23.   Journal of Cellular and Molecular Medicine(2020). Down‐regulation of RCC1 sensitizes immunotherapy by up‐regulating PD‐L1 via p27kip1/CDK4 axis in non‐small cell lung cancer. (IF:4.438 ) DOI: 10.1111/jcmm.16383

24.   Fish Shellfish Immunology(2020). Chlorpyrifos induces apoptosis and autophagy in common carp lymphocytes by influencing the TCR γ-dependent PI3K/AKT/JNK pathway. (IF:4.149) DOI: 10.1016/j.fsi.2020.02.039

25.   Journal of Biological Chemistry(2020). Estrogen-induced FOS-like 1 regulates matrix metalloproteinase e­xpression and the motility of human endometrial and decidual stromal cells. (IF:3.311 ) DOI: 10.1074/jbc.RA119.010701

26.   Journal of Fish Diseases(2020). Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ.  (IF:2.323) DOI: 10.1111/jfd.13138

27.   Chinese Medical Journal(2020). Topoisomerase II alpha promotes gallbladder cancer proliferation and metastasis through activating phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway.  (IF:1.585) DOI: 10.1097/CM9.0000000000001075

28.   Autophagy(2019). USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy. (IF:11.06 )  DOI: 10.1080/15548627.2019.1656957

29.   Oxidative Medicine and Cellular Longevity(2019). Aloin preconditioning attenuates hepatic ischemia/reperfusion injury via inhibiting TLR4/MyD88/NF-κB signal pathway in vivo and in vitro.  (IF:5.163 ) DOI: 10.1155/2019/3765898

30.   Oxidative Medicine and Cellular Longevity.(2019)Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite.(IF:5.076)DOI: 10.1155/2019/5769752

31.   Aging(2019). Circular RNA CircCCNB1 sponges micro RNA-449a to inhibit cellular senescence by targeting CCNE2. (IF:4.909 ) DOI: 10.18632/aging.102449

32.   International Journal of Biological Sciences(2019). Glycolytic enzyme PKM2 mediates autophagic activation to promote cell survival in NPM1-mutated leukemia. (IF:4.773) DOI: 10.7150/ijbs.30290

33.   Cancer Science(2019). NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma. (IF:4.751 ) DOI: 10.1111/cas.14190

34.   International Journal of Molecular Sciences(2019). Comparison of LncRNA e­xpression profiles during myogenic differentiation and adipogenic transdifferentiation of myoblasts. (IF:4.602) DOI: 10.3390/ijms20153725

35.   Biomed Pharmacother(2019). LncRNA MNX1-AS1 promotes progression of esophageal squamous cell carcinoma by regulating miR-34a/SIRT1 axis. (IF:3.743) DOI: 10.1016/j.biopha.2019.109029

36.   Mediators of Inflammation(2019). miR-23b negatively regulates sepsis-induced inflammatory responses by targeting ADAM10 in human THP-1 monocytes. (IF:3.545)  DOI: 10.1155/2019/5306541

37.   Frontiers in Physiology(2019). Survival motor neuron (SMN) protein insufficiency exacerbates renal ischemia/reperfusion injury. (IF:3.367 ) DOI: 10.3389/fphys.2019.00559

38.   International Immunopharmacology(2019). MiR-150 attenuates LPS-induced acute lung injury via targeting AKT3. (IF:3.361 ) DOI: 10.1016/j.intimp.2019.105794

39.   BioMed Research International(2019). PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-B Signaling Pathway.  (IF:2.366) DOI: 10.1155/2019/8650846

40.   Cancer Management and Research(2019). Long noncoding RNA FEZF1-AS1 promotes the motility of esophageal squamous cell carcinoma through Wnt/β-catenin pathway. (IF:2.243) DOI: 10.2147/CMAR.S196004

41.   Molecular Medicine Reports(2019). BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF_x005fκB signaling pathway in glomerular mesangial cells. (IF:2.085)  DOI: 10.3892/mmr.2019.10870

42.   Molecular Biology Reports(2019). Aberrant e­xpression of long noncoding RNAs in the serum and myocardium of spontaneous hypertensive rats. (IF:1.466 ) DOI: 10.1007/s11033-019-05086-x

43.   Journal of Experimental & Clinical Cancer Research(2018). INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia. (IF:5.189 ) DOI: 10.1186/s13046-018-0675-9

44.   Journal of Endocrinology(2018). SRT2104 attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53. (IF:4.706 ) DOI: 10.1530/JOE-17-0672

45.   Biochimica et Biophysica Acta (BBA) - Molecular Cell Research(2018). MDM2 controls NRF2 antioxidant activity in prevention of diabetic kidney disease. (IF:3.982)  DOI: 10.1016/j.bbamcr.2018.04.011

46.   Oncotarget(2018). FC-99 ameliorates sepsis-induced liver dysfunction by modulating monocyte/macrophage differentiation via Let-7a related monocytes apoptosis. (IF:3.765)    DOI: 10.18632/oncotarget.24127

47.   Oncotarget(2018). Metformin synergistically suppress tumor growth with doxorubicin and reverse drug resistance by inhibiting the e­xpression and function of P-glycoprotein in MCF7/ADR cells and xenograft models. (IF:3.765) DOI: 10.18632/oncotarget.23187

48.   Biochemistry and Cell Biology(2018). MMS19 localizes to mitochondria and protects the mitochondrial genome from oxidative damage. (IF:2.25 ) DOI: 10.1139/bcb-2017-0149

49.   Biochimica et Biophysica Acta(2018). A novel brown adipocyte-enriched long non-coding RNA that is required for brown adipocyte differentiation and sufficient to drive thermogenic gene program in white adipocytes. (IF:2.014 ) DOI: 10.1016/j.bbalip.2018.01.008

50.   Cell Research(2017). 5-methylcytosine promotes mRNA export — NSUN2 as the  methyltransferase and ALYREF as an m5C reader. (IF:13.9 ) DOI: 10.1038/cr.2017.55

51.   Free Radical Biology & Medicine(2017). Epigallocatechin gallate upregulates NRF2 to prevent diabetic nephropathy via disabling KEAP1. (IF:6.078 ) DOI: 10.1016/j.freeradbiomed.2017.04.365

52.   Journal of Molecular Endocrinology(2017). SP600125 suppresses Keap1 e­xpression and results in NRF2-mediated prevention of diabetic nephropathy. (IF:3.577) DOI: 10.1530/JME-17-0260

53.   Oncotarget(2016). Knockdown of Golgi phosphoprotein 2 inhibits hepatocellular carcinoma cell proliferation and motility. (IF:3.765 ) DOI: 10.18632/oncotarget.7271

54.   Oncotarget(2016). Migration-inducing gene 7 promotes tumorigenesis and angiogenesis and independently predicts poor prognosis of epithelial ovarian cance. (IF:3.765) DOI: 10.18632/oncotarget.8487

55.   Molecular Nutrition Food Research(2014). Low‐dose diet supplement of a natural flavonoid, luteolin, ameliorates diet‐induced obesity and insulin resistance in mice. (IF:5.426)  DOI: 10.1002/mnfr.201300830

56.   scientific reports(2013). APG: an active protein-gene network model to quantify regulatory signals in complex biological systems. (IF:4.149) DOI: 10.1038/srep01097


相关产品链接

RFectPM小核酸转染试剂(国际专利技术,针对原代细胞siRNA、antisenseRNA、microRNA等转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=20

RFectSP小核酸转染试剂(国际专利技术,针对悬浮细胞siRNA、antisenseRNA、microRNA等转染

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=158

RFect质粒DNA转染试剂盒(新产品!国际专利技术, 针对培养细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=167

RFectPM原代细胞DNA转染试剂盒(新产品!国际专利技术, 针对原代细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=189







分享到:
常州百代生物科技股份有限公司 常州市戚墅堰东方东路51号新东方物流园 网站地图
电话:86-0519-88050392-607/606/635 监督电话:15902199991 传真:0519-83382790 QQ:2728573193、2790565395、2257231185微信:18918859070
邮箱:czbdswkj@163.com 备案号:苏ICP备14052941号
在线客服在线客服
离线留言
在线业务咨询
王经理
座机:0519-88050392-607
qq2728573193
点击这里给我发消息
微信号18918859070
朱经理
座机:0519-88050392-635
qq2257231185
点击这里给我发消息
微信号18118014141
吴经理
座机:0519-88050392-606
qq2790565395
点击这里给我发消息
微信号18921004001
在线服务时间
周一至周五(工作日)
8:30-17:30
其他时间请联系微信