RFect^PMsiRNA/miRNA Transfection Reagent

RFect^PM原代细胞小核酸转染试剂

‍‍产品简介

RFect^PM原代细胞小核酸转染试剂是我公司美国研发团队在RFect细胞株小核酸转染试剂的基础上加以改进研发成功的专门用于原代细胞小核酸转染的试剂。RFect^PM可用来转染siRNA、miRNA mimics、miRNA inhibitor等200bp以内的小分子RNA，可转染绝大多数原代细胞。目前，无论国外还是国内，原代细胞的核酸转染都是个热点难题，市场还缺乏真正有效的商品化的原代细胞核酸转染试剂。RFect^PM能够高效转染绝大多数原代细胞，获得比较理想的基因敲除效果。甚至对一些活体寄生虫幼虫，RFect^PM也能获得较为理想的转染结果。对于大多数原代细胞，RFect^PM的细胞转染阳性率都在80%以上，而转染细胞死亡率不到10%。RFect^PM的使用也极其简便，先将sRNA与RFect^PM室温混合，再将siRNA-RFect^PM混合物直接加入含培养基的细胞，血清对转染效果没有影响，不必刻意添加或更换培养液。

产品特点‍

· 原代细胞转染性能：细胞转染阳性率一般在80%以上，基因敲除效果明显，肝细胞Lamin A/C 基因敲除效率在95%以上；

·极低的细胞毒性：转染细胞死亡率不到10%，大大降低了因细胞毒性对实验结果的影响；

· 转染细胞范围广，绝大多数原代细胞都能获得比较理想的转染结果。

Fig.1. Superior knockdown with RFectPM transfection reagent on difficult transfection primary cells.Differentprimarycells were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFectPM.In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig.2. RFectPM transfection reagent offers high level of gene knockdown with low concentration of siRNA. Lamin A/C siRNA-RFectPM transfection complexes were prepared in 96-well plates,and then bovine chondrocytes were added to each well.

适用细胞

Clone 9（大鼠肝细胞），HUVEC（人脐静脉原代内皮细胞），原代血管平滑肌细胞，大鼠原代心肌细胞，Primary Human Fibroblasts（人原代成纤维细胞），大鼠骨髓间充质干细胞，MSC（间充质干细胞），BE(2)C（人神经母细胞瘤细胞），MRC-5（人胚胎成纤维细胞），NIH3T3（小鼠胚胎成纤维细胞），B16F10（小鼠黑色素瘤高转移细胞），BNL.CL2（小鼠胚胎肝细胞），C6（大鼠神经胶质瘤细胞），神经元细胞等。

相关文献

1.  Basic Research in Cardiology(2021). HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype. (IF:11.981) DOI: 10.1007/s00395-021-00867-7

2.   NUCLEIC ACIDS RESEARCH(2021). XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage. (IF:11.24 ) DOI: 10.1093/nar/gkv472

3.  MYO1F regulates antifungal immunity by regulating acetylation of microtubules. (IF:11.205) DOI: 10.1073/pnas.2100230118

4.  Cellular & Molecular Immunology(2021). The miR-628-5p Facilitates Enterovirus 71 Infection by Suppressing TRAF3-Mediated p65 and IRF3 Signaling. (IF:8.484) DOI: 10.1038/s41423-020-0453-4

5.  Levobupivacaine Induces Ferroptosis by miR-489-3p/SLC7A11 Signaling in Gastric Cancer. (IF:5.81) DOI: 10.3389/fphar.2021.681338

6.  Proteome Analysis in PAM Cells Reveals That African Swine Fever Virus Can Regulate the Level of Intracellular Polyamines to Facilitate Its Own Replication through ARG1. (IF:5.046) DOI: 10.3390/v13071236

7.  AGING-US(2021). Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury. (IF:4.909 ) DOI: 10.18632/aging.202611

8.  International Journal of Biological Sciences(2021). DGCR5 Promotes Gallbladder Cancer by Sponging MiR-3619-5p via MEK/ERK1/2 and JNK/p38 MAPK Pathways. (IF:4.773) DOI: 10.7150/jca.46351

9.  International Journal of Biological Sciences(2021). Rac GTPase activating protein 1 promotes gallbladder cancer via binding DNA ligase 3 to reduce apoptosis. (IF:4.773) DOI: 10.7150/ijbs.58857

10.  International Journal of Biological Sciences(2021). ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. (IF:4.773)  DOI:10.7150/ijbs.57623

11.  International Journal of Biological Sciences(2021). KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. (IF:4.773 ) DOI: 10.7150/ijbs.54074

12.  International Journal of Biological Sciences(2021). DGCR5 is activated by PAX5 and promotes pancreatic cancer via targeting miR-3163/TOP2A and activating Wnt/β-catenin pathway. (IF:4.773 ) DOI: 10.7150/ijbs.55636

13.  Journal of Cellular and Molecular Medicine(2021). Down‐regulation of RCC1 sensitizes immunotherapy by up‐regulating PD‐L1 via p27kip1/CDK4 axis in non‐small cell lung cancer. (IF:4.438) DOI: 10.1111/jcmm.16383

14.  scientific reports(2021). Clinical significance of Annexin A2 e­xpression in oral squamous cell carcinoma and its influence on cell proliferation, migration and invasion. (IF:4.149) DOI: 10.1038/s41598-021-84675-y

15.  Journal of Cancer(2021). LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1. (IF:3.565) DOI: 10.7150/jca.51136

16.  Environmental Geochemistry and Health(2021). Inorganic arsenic influences cell apoptosis by regulating the e­xpression of MEG3 gene. (IF:3.472 ) DOI: 10.1007/s10653-020-00740-x

17.  Bta-miR-223 Targeting CBLB Contributes to Resistance to Staphylococcus aureus Mastitis Through the PI3K/AKT/NF-κB Pathway. (IF:3.412) DOI: 10.3389/fvets.2020.00529

18.  Molecular Human Reproduction(2021). Excess fibronectin 1 participates in pathogenesis of pre-eclampsia by promoting apoptosis and autophagy in vascular endothelial cells. (IF:3.469) DOI: 10.1093/molehr/gaab030

19.  American Journal of Translational Research(2021). Irg1-itaconate axis protects against acute kidney injury via activation of Nrf2. (IF:3.375 )

20.  ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2021). Arsenic exposure increased e­xpression of HOTAIR and LincRNA-p21 in vivo and vitro. (IF:3.33) DOI: 10.1007/s11356-020-10487-8

21.  Environmental Toxicology and Pharmacology(2021). Long non-coding RNA DICER1-AS1-low e­xpression in arsenic-treated A549 cells inhibits cell proliferation by regulating the cell cycle pathway. (IF:3.292 ) DOI: 10.1016/j.etap.2021.103617

22.  ENVIRONMENTAL TOXICOLOGY(2021). Inorganic arsenic‐mediated upregulation of AS3MT promotes proliferation of nonsmall cell lung cancer cells by regulating cell cycle genes. (IF:3.118 ) DOI: 10.1002/tox.23026

23.  Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells. (IF:2.59) DOI: 10.1590/1414-431x2021e11156

24.  FEBS Open Bio(2021). SREBP1/FASN/cholesterol axis facilitates radioresistance in colorectal cancer.  (IF:2.231 ) DOI: 10.1002/2211-5463.13137

25.  Clinical And Translational Medicine(2020). Long noncoding RNA lncGALM increases risk of liver metastasis in gallbladder cancer through facilitating N‐cadherin and IL‐1β‐dependent liver arrest and tumor extravasation. (IF:7.919 ) DOI: 10.1002/ctm2.201

26.  Clinical And Translational Medicine(2020). Mir‐27a‐3p attenuates bronchiolitis obliterans in vivo via the regulation of dendritic cells' maturation and the suppression of myofibroblasts' differentiation. (IF:7.919 ) DOI: 10.1002/ctm2.140

27.  Journal of Experimental & Clinical Cancer Research(2020). Loss of miR-192-5p initiates a hyperglycolysis and stemness positive feedback in hepatocellular carcinoma. (IF:7.068) DOI: 10.1186/s13046-020-01785-7

28.  American Journal of Cancer Research(2020). ERRα promotes pancreatic cancer progression by enhancing the transcription of PAI1 and activating the MEK/ERK pathway.  (IF:5.884 )

29.   Frontiers in Cell and Developmental Biology(2020). CRLF1 is a key regulator in the ligamentum flavum hypertrophy. (IF:5.8 ) DOI: 10.3389/fcell.2020.00858

30.   Cancer Science(2020). Anthrax toxin receptor 1/tumor endothelial marker 8 promotes gastric cancer progression through activation of the PI3K/AKT/mTOR signaling pathway. (IF:4.985) DOI: 10.1111/cas.14326

31.  Journal of Cellular and Molecular Medicine(2020). Down‐regulation of RCC1 sensitizes immunotherapy by up‐regulating PD‐L1 via p27kip1/CDK4 axis in non‐small cell lung cancer. (IF:4.438 ) DOI: 10.1111/jcmm.16383

32.  Frontiers in Pharmacology(2020). Long Noncoding RNA LINC-PINT Suppresses Cell Proliferation, Invasion, and EMT by Blocking Wnt/β-Catenin Signaling in Glioblastoma. (IF:4.225) DOI: 10.3389/fphar.2020.586653

33.  Fish Shellfish Immunology(2020). Chlorpyrifos induces apoptosis and autophagy in common carp lymphocytes by influencing the TCR γ-dependent PI3K/AKT/JNK pathway. (IF:4.149) DOI: 10.1016/j.fsi.2020.02.039

34.  Journal of Biological Chemistry(2020). Estrogen-induced FOS-like 1 regulates matrix metalloproteinase e­xpression and the motility of human endometrial and decidual stromal cells. (IF:3.311 ) DOI: 10.1074/jbc.RA119.010701

35.  Journal of Fish Diseases(2020). Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ.  (IF:2.323) DOI: 10.1111/jfd.13138

36.  Molecular Medicine Reports(2020). Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3_x005f_x001e_E1 cells via an autocrine mechanism. (IF:2.085) DOI: 10.3892/mmr.2020.11506

37.  Chinese Medical Journal(2020). Topoisomerase II alpha promotes gallbladder cancer proliferation and metastasis through activating phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway.  (IF:1.585) DOI: 10.1097/CM9.0000000000001075

38.  Autophagy(2019). USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy. (IF:11.06 )  DOI: 10.1080/15548627.2019.1656957

39.  Oxidative Medicine and Cellular Longevity(2019). Aloin preconditioning attenuates hepatic ischemia/reperfusion injury via inhibiting TLR4/MyD88/NF-κB signal pathway in vivo and in vitro.  (IF:5.163 ) DOI: 10.1155/2019/3765898

40.  Oxidative Medicine and Cellular Longevity.(2019)Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite.(IF:5.076)DOI: 10.1155/2019/5769752

41.  Aging(2019). Circular RNA CircCCNB1 sponges micro RNA-449a to inhibit cellular senescence by targeting CCNE2. (IF:4.909 ) DOI: 10.18632/aging.102449

42.  International Journal of Biological Sciences(2019). Glycolytic enzyme PKM2 mediates autophagic activation to promote cell survival in NPM1-mutated leukemia. (IF:4.773) DOI: 10.7150/ijbs.30290

43.  Cancer Science(2019). NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma. (IF:4.751 ) DOI: 10.1111/cas.14190

44.  International Journal of Molecular Sciences(2019). Comparison of LncRNA e­xpression profiles during myogenic differentiation and adipogenic transdifferentiation of myoblasts. (IF:4.602) DOI: 10.3390/ijms20153725

45.  Biomed Pharmacother(2019). LncRNA MNX1-AS1 promotes progression of esophageal squamous cell carcinoma by regulating miR-34a/SIRT1 axis. (IF:3.743) DOI: 10.1016/j.biopha.2019.109029

46.  Mediators of Inflammation(2019). miR-23b negatively regulates sepsis-induced inflammatory responses by targeting ADAM10 in human THP-1 monocytes. (IF:3.545)  DOI: 10.1155/2019/5306541

47.  Frontiers in Physiology(2019). Survival motor neuron (SMN) protein insufficiency exacerbates renal ischemia/reperfusion injury. (IF:3.367 ) DOI: 10.3389/fphys.2019.00559

48.  International Immunopharmacology(2019). MiR-150 attenuates LPS-induced acute lung injury via targeting AKT3. (IF:3.361 ) DOI: 10.1016/j.intimp.2019.105794

49.  Eur Rev Med Pharmacol Sci(2019). Long noncoding RNA PCAT6 regulates cell growth and metastasis via Wnt/beta-catenin pathway and is a prognosis marker in cervical cancer. (IF:2.727) DOI: 10.26355/eurrev_201903_17233

50.  BioMed Research International(2019). PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-���B Signaling Pathway.  (IF:2.366) DOI: 10.1155/2019/8650846

51.  Cancer Management and Research(2019). Long noncoding RNA FEZF1-AS1 promotes the motility of esophageal squamous cell carcinoma through Wnt/β-catenin pathway. (IF:2.243) DOI: 10.2147/CMAR.S196004

52.  Molecular Medicine Reports(2019). BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF_x005fκB signaling pathway in glomerular mesangial cells. (IF:2.085)  DOI: 10.3892/mmr.2019.10870

53.  Molecular Biology Reports(2019). Aberrant e­xpression of long noncoding RNAs in the serum and myocardium of spontaneous hypertensive rats. (IF:1.466 ) DOI: 10.1007/s11033-019-05086-x

54.  Journal of Experimental & Clinical Cancer Research(2018). INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia. (IF:5.189 ) DOI: 10.1186/s13046-018-0675-9

55.  Journal of Endocrinology(2018). SRT2104 attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53. (IF:4.706 ) DOI: 10.1530/JOE-17-0672

56. scientific reports(2018). Sodium selenite inhibits deoxynivalenol-induced injury in GPX1-knockdown porcine splenic lymphocytes in culture. (IF:4.149) DOI: 10.1038/s41598-018-36149-x

57.  Biochimica et Biophysica Acta (BBA) - Molecular Cell Research(2018). MDM2 controls NRF2 antioxidant activity in prevention of diabetic kidney disease. (IF:3.982)  DOI: 10.1016/j.bbamcr.2018.04.011

58.  Oncotarget(2018). FC-99 ameliorates sepsis-induced liver dysfunction by modulating monocyte/macrophage differentiation via Let-7a related monocytes apoptosis. (IF:3.765)    DOI: 10.18632/oncotarget.24127

59.  Oncotarget(2018). Metformin synergistically suppress tumor growth with doxorubicin and reverse drug resistance by inhibiting the e­xpression and function of P-glycoprotein in MCF7/ADR cells and xenograft models. (IF:3.765) DOI: 10.18632/oncotarget.23187

60.  Biochemistry and Cell Biology(2018). MMS19 localizes to mitochondria and protects the mitochondrial genome from oxidative damage. (IF:2.25 ) DOI: 10.1139/bcb-2017-0149

61.  Biochimica et Biophysica Acta(2018). A novel brown adipocyte-enriched long non-coding RNA that is required for brown adipocyte differentiation and sufficient to drive thermogenic gene program in white adipocytes. (IF:2.014 ) DOI: 10.1016/j.bbalip.2018.01.008

62.  Cell Research(2017). 5-methylcytosine promotes mRNA export — NSUN2 as the  methyltransferase and ALYREF as an m⁵C reader. (IF:13.9 ) DOI: 10.1038/cr.2017.55

63.  Free Radical Biology & Medicine(2017). Epigallocatechin gallate upregulates NRF2 to prevent diabetic nephropathy via disabling KEAP1. (IF:6.078 ) DOI: 10.1016/j.freeradbiomed.2017.04.365

64.  Journal of Molecular Endocrinology(2017). SP600125 suppresses Keap1 e­xpression and results in NRF2-mediated prevention of diabetic nephropathy. (IF:3.577) DOI: 10.1530/JME-17-0260

65.  Oncotarget(2016). Knockdown of Golgi phosphoprotein 2 inhibits hepatocellular carcinoma cell proliferation and motility. (IF:3.765 ) DOI: 10.18632/oncotarget.7271

66.  Oncotarget(2016). Migration-inducing gene 7 promotes tumorigenesis and angiogenesis and independently predicts poor prognosis of epithelial ovarian cance. (IF:3.765) DOI: 10.18632/oncotarget.8487

67.  Molecular Nutrition Food Research(2014). Low‐dose diet supplement of a natural flavonoid, luteolin, ameliorates diet‐induced obesity and insulin resistance in mice. (IF:5.426)  DOI: 10.1002/mnfr.201300830

68.  scientific reports(2013). APG: an active protein-gene network model to quantify regulatory signals in complex biological systems. (IF:4.149) DOI: 10.1038/srep01097

相关产品链接

RFect细胞株小核酸转染试剂（国际专利技术，针对贴壁细胞siRNA、antisenseRNA、microRNA等转染）

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=19

RFect^SP小核酸转染试剂（国际专利技术，针对悬浮细胞siRNA、antisenseRNA、microRNA等转染）

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=158

RFect质粒DNA转染试剂盒（新产品！国际专利技术， 针对培养细胞质粒DNA转染）

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=167

RFect^PM原代细胞质粒DNA转染试剂盒(新产品！国际专利技术， 针对原代细胞质粒DNA转染)

http://www.biodai.com.cn/show.asp?typeid=6&sortid=55&id=189